Description

Biolaxi Enzymes is one of the leading manufacturers and suppliers of enzymes, operating since 2007. We offer a broad range of high-quality enzymes, including trypsin enzymes, which are widely used in industrial, commercial, and biomedical applications.

Trypsin enzymes are produced through microbial fermentation. Microbial production, particularly using Streptomyces griseus trypsin (SGT), offers advantages such as scalability, consistent quality, and a reduced risk of contaminants. SGT can be expressed in various microorganisms, including Escherichia coli, Bacillus subtilis, and Pichia pastoris, enabling cost-effective and sustainable large-scale production.

Technical Specifications

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Mechanism

Trypsin is a serine protease that catalyzes the hydrolysis of peptide bonds, specifically cleaving at the carboxyl side of lysine (K) and arginine (R) residues. This proteolytic activity breaks down complex proteins into smaller peptides or amino acids, facilitating digestion and protein analysis. The enzyme operates optimally in alkaline conditions (pH 7.0 – 9.0) and requires calcium ions for stability and activity. Its mechanism involves a catalytic triad (serine, histidine, and aspartate) that enables precise and efficient protein cleavage.

Isozymes

Trypsin enzymes exist in multiple isozyme forms, each with slight variations in structure and function. These isozymes are categorized based on their source and specific activity profiles:

Trypsin-1 (Cationic Trypsin):

• Predominant form found in the pancreas.
• High stability and activity in alkaline environments.
• Commonly used in biotechnological applications due to its robust performance.

Trypsin-2 (Anionic Trypsin):

• Less abundant but exhibits higher catalytic efficiency in certain conditions.
• Often used in specialized research applications requiring precise protein digestion.

Trypsin-3 (Mesotrypsin):

• A minor variant with unique substrate specificity.
• Resistant to natural protease inhibitors, making it valuable in specific diagnostic and therapeutic applications.

Applications

Pharmaceuticals and Biotechnology:

• Cell Culture: Used for dissociating adherent cells from culture surfaces, enabling subculturing and downstream analysis.
• Protein Digestion: Facilitates the breakdown of proteins for peptide mapping and mass spectrometry analysis.
• Drug Development: Supports the production of biologics and vaccines by processing recombinant proteins.

Food Industry:

• Protein Hydrolysis: Enhances the digestibility and nutritional value of protein-rich foods, such as infant formula and sports nutrition products.
• Flavor Enhancement: Breaks down proteins to release amino acids, improving flavor profiles in processed foods.

Research and Diagnostics:

• Proteomics: Essential for protein identification and characterization in research studies.
• Diagnostic Kits: Used in immunoassays and diagnostic tests for disease detection and biomarker analysis.

Industrial Applications:

• Leather Processing: Aids in dehairing and bating during leather production.
• Waste Management: Breaks down proteinaceous waste in environmental cleanup processes.

Therapeutic and Clinical Uses:

• Inflammatory Conditions: Used in combination with bromelain and rutosides to treat inflammation.
• Wound Healing: Applied directly to wounds, including mouth ulcers, to remove dead tissue and promote healthy tissue growth.

Certificates

• Halal Certificate
• ISO 9001:2015
• cGMP
• Food and Drugs Administration
• GMP Certificate
• Kosher Certificate

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